Higher Order Structure Determination of Biomolecules by Circular Dichroism
Circular Dichroism (CD) is a powerful method to determine higher order protein structure. A circular dichroism spectropolarimeter measures left and right circularly polarized light of optically active molecules. It is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer.
Our JASCO J-810 circular dichroism instrument can scan from the near UV (163 nm) to visible wavelengths (900 nm). The instrument has automated temperature control and titration accessories for accurately and rapidly completing scans.
- Determination of the native conformation of biomolecules (helical, pleated, random).
- Detection of changes in protein conformation (folding/unfolding) by controlled changes in salt, pH, and buffer conditions.
- Thermal stability of proteins during forward and reverse melting (denaturation and renaturation).
- Determination of changes in the secondary structure of a protein or polypeptide caused by ligand binding.
- Generation of guanidinium hydrochloride melt curves.
- Analysis of the purity and stability of optically active substances of different batches.
- Preliminary stability testing of proteins before NMR analysis.
Our analytical scientists can assist with sample preparation and consult on data interpretation to achieve optimal results.
Email Info@FinaBio.com to learn more about our analytical services