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Fina Biosolutions now offers CRM197 pre-labeled with bromoacetate, allowing for the easy conjugation of thiolated antigens.  FinaBio’s CRM197, EcoCRM®, is labeled with ~15 bromoacetate groups.  The labeled protein is provided frozen, ready to thaw and conjugate to a thiol-containing antigen.

Examples of antigens that have been conjugated to CRM-bromoacetate include cysteine-peptides, thiol-labeled oligosaccharides, polysaccharides and proteins.  Depending on the nature of the antigen, gel filtration or spin filters can be used to purify the conjugate.


~15 Bromoacetate/CRM197
5 mg/ml
PBS + 5% sucrose, pH 6.8
Store at <-20°C

Note:  Use within 1 hr after thawing.  Note that CRM197 will denature in acidic buffers.  Care should be taken to keep the conjugation pH between 6.8 and 9 for CRM-bromoacetate.  We recommend familiarity with thiol-ether conjugation.  See GT Hermanson, Bioconjugate Techniques.

Size Price
1 mg $375
5 mg $875
10 mg $1,500
25 mg $3,750
50 mg $6,750
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