Protein A has high affinity to IgG from various species, for instance human, rabbit and guinea pig but only weak interaction with bovine and mouse. It binds with the Fc region of antibodies through interaction with the heavy chain. The Oncosimis® recombinant protein A consists of only 251 amino acids and has a predicted molecular mass of 28 kDa as estimated by SDS-PAGE.

The recombinant protein A is produced by expressing a modified protein A gene in E. coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98%. No human IgG affinity step is used during validated fermentation and purification and it is devoid of bacterial contaminants found normally in native Protein A.

Applications: Powerful affinity ligand for monoclonal antibody purification
Suitable for various immunochemical assays including WB, IHC, IP, and ELISA